Despite the profound biological insights revealed by proteomic and metabolomic technologies they still fail to reveal the full function of many small molecules and proteins found in nature. Our lab is developing new isobaric tagging based methodologies in quantitative proteomics and metabolomics to uncover metabolite and protein function^1,2,3.

1. Murphy JP, Yu Q, Konda P, Paulo JA, Jedrychowski MP, Kowalewski DJ, Schuster H, Kim Y, Clements D, Jain A, Stevanovic S, Gygi SP, Mancias JD, Gujar S. (2019). Multiplexed relative quantitation with isobaric tagging mass spectrometry reveals class I major histocompatibility complex ligand dynamics in response to doxorubicin. Anal. Chem. 91(8): 5106-5115.
2. Murphy JP, Stepanova E, Everley RE, Paulo JA, Gygi SP. (2015). Comprehensive temporal protein dynamics during the diauxic shift in Saccharomyces cerevisiae. Mol. Cell. Proteomics. 14:2454-2465.
3. Murphy JP, Everley RE, Coloff JL, Gygi SP. (2014). Combining amine metabolomics and quantitative proteomics of cancer cells using derivatization with isobaric tags. Anal. Chem.86: 3585-3593.